Copyright © 1990 by the European Society of Cardiology.
© 1990 The European Society of Cardiology
Alterations of the mitochondrial respiratory chain in human dilated cardiomyopathy
Department of Cardiology, University of Göttingen F.R.G.
Received 12 January 1989; revised 27 June 1989; .
Address for Correspondence: Dr. A. Buchwald, Dept of Cardiology, University Clinic, Robert-Koch-Str. 40, 3400 Görriugen, West Germany.
Abstract
The defects underlying the impairment of systolic pump function in human dilated cardiomyopathy (DCM) are not known. We isolated mitochondrial particles from 10 hearts of transplant recipients with DCM and from nine normal hearts not used for transplantation. Yield was similar in both groups (2.81 mg mitochondrialprotein pergram heart). Cytochrome content (difference spectrophotometry ) was found reduced in DCM mitochondria, e.g. cytochrome c was 0.295 ±0.06 in the DCM group and 0.371 ±0.04 funolgM–1he control group (P/0.05). Enzymatic activity of the cytochrome-containing complexes III (3.77±0.82 vs 4.95±1.15 fimol min–1 mg–1) and IV (2.63±0.96 vs 3.65 ± 0.6 nmol min–1.mg–1) of the respiratory chain was reduced in the DCM group (P / 0.05). Complex IV, the cytochrome c oxidase, in the DCM group showed impaired activity also in whole heart homogenates (0.173 ±0.04 vs 0.258 ± 0.8 µmol min–1.mg–1). Subunit composition of the cytochrome c oxidase on sodium dodecyl sulphate–gel electrophoresis did not differ between DCM and normal hearts. Activity of complexes II and V of the respiratory chain, not containing cytochromes, was unchanged in DCM mitochondria compared with the control group. The present data show a decrease in cytochrome content and in cytochrome-dependent enzyme activity in human dilated cardiomyopathy. Further studies are necessary to clarify whether these findings are specific for dilated cardiomyopathy or whether they are epiphenomena of failing hearts.
Key Words: Human dilated cardiomyopathy • mitochondria • cytochromes • respiratory chain
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