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European Heart Journal 1990 11(Supplement E):128-133; doi:10.1093/eurheartj/11.suppl_E.128
Copyright © 1990 by the European Society of Cardiology.
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© 1990 The European Society of Cardiology

Lipoprotein metabolism of human and rabbit arterial cells in primary culture

O. Jaakkola, O.-P. Kallioniemi and T. Nikkari

Department of Biomedical Sciences, University of Tampere Tampere, Finland

Address for correspondence: Olli Jaakkola, Department of Biomedical Sciences, University of Tampere, P.O. Box 607, SF-33101 Tampere, Finland

The early atherosclerotic lesion, the fatty streak, consists of cholesteryl ester-containing foam cells originating mainly from monocyte-macrophages and to a lesser extent from smooth muscle cells. In this study, we describe lipoprotein uptake and cholesterol accumulation into enzyme-dispersed primary cell cultures from cholesterolfed rabbit aortas and human aortic fatty streaks. Uptake of fluorescently labelled acetylated low density lipoprotein (acetyl-LDL) was demonstrable in macrophage-derived foam cells and in many smooth muscle cells in early primary cultures. The uptake of acetyl-LDL led to significantly enhanced cellular esterification of cholesterol. Fluorescent β-migrating very low density lipoprotein (β-VLDL) was internalized by a considerable number of lesion macrophages and also by smooth muscle cells. Also β-VLDL uptake stimulated cholesterol esterification, although the effect was milder than that of acetyl-LDL. These findings lend support to the assumption that, during atherogenesis, arterial macrophages are capable of accumulating cholesteryl esters by receptor-mediated uptake of β-VLDL and modified LDL. The internalization of modified LDL by smooth muscle cells represents a mechanism potentially contributing to the formation of foam cells in the atherosclerotic lesion.

Key Words: Atherosclerosis • macrophages • smooth muscle cells • lipoproteins • β-low density lipoprotein • low density lipoprotein


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H. S. Kruth, W. Huang, I. Ishii, and W.-Y. Zhang
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[Abstract] [Full Text] [PDF]


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