Skip Navigation

European Heart Journal 1990 11(Supplement E):88-99; doi:10.1093/eurheartj/11.suppl_E.88
Copyright © 1990 by the European Society of Cardiology.
This Article
Right arrow Full Text (PDF)
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ylä-Herttuala, S.
Right arrow Articles by Witztum, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ylä-Herttuala, S.
Right arrow Articles by Witztum, J. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1990 The European Society of Cardiology

Lipoproteins in normal and atherosclerotic aorta

S. Ylä-Herttuala, W. Palinski, M. E. Rosenfeld, D. Steinberg and J. L. Witztum

Department of Medicine, University of California San Diego, CA, USA.

Address for correspondence: Seppo Ylä-Herttuala, M.D., Department of Medicine, M-013D university of California, San Diego, La Jolla, CA 92093-0613, U.S.A.

Each method used for the extraction and isolation of intimal lipoproteins has advantages and disadvantages. Gentle extraction methods are needed to characterize subtle modifications in the structure and biologic properties of the lipoproteins, whereas more aggressive methods are needed if the goal is to maximize the yield of lipoproteins from atherosclerotic arteries. The present paper evaluates different methods used for the isolation of intima1 lipoproteins.

Normal intima contains remnant-like and low density lipoprotein (LDL)-like particles that more strongly stimulate cholesterol esterification in macrophages than do control plasma LDL. Both fractions contain apolipoprotein (apo) E but neither shows clear signs of oxidative modification.

LDL-like particles from atherosclerotic lesions, on the other hand, contain malondialdehyde- and 4- hydroxynonenal-lysine adducts in apo B, are chemotactic for monocytes and show increased degradation in macrophages, a process that oxidized LDL prepared in vitro can compete with. The findings support the conclusion that at least a portion of the LDL isolated from atherosclerotic lesions is similar, if not identical, to oxidatively modified LDL.

Key Words: Atherosclerosis • density gradient ultracentrifugation • isolation and characterization of intimal lipoproteins • monoclonal antibodies • oxidatively modified LDL


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
H.-C. Ou, W.-J. Lee, I-T. Lee, T.-H. Chiu, K.-L. Tsai, C.-Y. Lin, and W. H.-H. Sheu
Ginkgo biloba extract attenuates oxLDL-induced oxidative functional damages in endothelial cells
J Appl Physiol, May 1, 2009; 106(5): 1674 - 1685.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
K. Nishi, H. Itabe, M. Uno, K. T. Kitazato, H. Horiguchi, K. Shinno, and S. Nagahiro
Oxidized LDL in Carotid Plaques and Plasma Associates With Plaque Instability
Arterioscler Thromb Vasc Biol, October 1, 2002; 22(10): 1649 - 1654.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.