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European Heart Journal 1991 12(Supplement D):32-35; doi:10.1093/eurheartj/12.suppl_D.32
Copyright © 1991 by the European Society of Cardiology.
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© 1991 The European Society of Cardiology

Diagnostic assessment of macrophage phenotypes in cardiac transplant biopsies

B. Mues*, B. Brisse{dagger}, G. Steinhoff{ddagger}, T. Lynn*, T. Hewett*, C. Sorg||, N. Zuhdi* and G. Robbins*,

* Oklahoma Transplantation Institute, Baptist Medical Center Oklahoma City, U.S.A.
{dagger} Department of Cardiology, University of Muenster Germany
{ddagger} Institute of Experimental Dermatology, University of Muenster Germany
|| Medizinische Hochschute, Hannover, Department of Transplantation Surgery Germany

Address for correspondence: Dr Galen Robbins, Oklahoma Transplantation Institute, Baptist Medical Center of Oklahoma, 3300 N.W Expressway, Oklahoma City, OK 732112, U.S.A.

Forty-one endomyocardial biopsies of the right interventricular septum have been investigated in 24 immunosuppressed patients after orthotopic heart transplantation. Monoclonal antibodies 27E10, 25F9, and RM3/1, which react with different macrophage phenotypes, and antisera MRP-8 and MRP-14, specific for proteins expressed on endothelial and monocyte cell surfaces in inflammation as well as markers for CD4+ and CD8+ T-lymphocytes, were employed in an indirect immunoperoxidase staining technique. This methodology permits more physiological recognition of the inflammatory process within the myocardium. It was possible to verify and to distinguish acute early, late and down-regulatory stages of inflammation in 33 biopsies (80%). No evidence of inflammation was found in seven biopsies (17%). Conventional histopathology with haemaloxylin-eosin and Masson's trichrome was performed simultaneously, and demonstrated inflammation to be present in 23 of 41 biopsies (56%).

An important finding is that CD4+ and CD8+ lymphocytes were absent in 15 of 41 specimens (37%) although there was inflammation proven by the presence of different macrophage phenotypes. The results indicate the necessity of long-term serial investigations of the physiological role of specific inflammatory macrophage phenotypes during the rejection process. It is concluded that the phenotyping of macrophage and endothelial cell differentiation antigens offers a sensitive approach to assess diagnosis of myocardial inflammation as a consequence of ongoing rejection in cardiac allografts.

Key Words: Acute and chronic rejection • inflammation • macrophage phenotypes


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