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European Heart Journal 1991 12(Supplement F):132-134; doi:10.1093/eurheartj/12.suppl_F.132
Copyright © 1991 by the European Society of Cardiology.
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© 1991 The European Society of Cardiology

Immunocytochemical localization of G-proteins ({alpha} subunits) in rat heart tissue

W. Schulze, A. Kössler, K. D. Hinsch*, W. Rosenthal*, L. Will-Shahab, I. Küttner, T. Rada{dagger}, M. Vannauer and H. Breter

Institute of Cardiovascular Research Berlin-Buch
* Institute of Pharmacology, Free University Berlin
{dagger} Institute of Heart Research, Slovak Academy of Sciences Bratislava

Correspondence: Dr W Schultz, Institute of Cardiovascular Research, Robert-Rössle-Str. 10, 0-1115 Berlin, Germany.

The subcellular localization of peptide antibodies against G-protein {alpha} subunits was studied by the indirect immunogold technique with Lowicryl K4M embedded rat cardiac tissue. Two antibodies were used. The {alpha}common peptide antibody recognizes the {alpha} subunits of G1, G1 and Go. The {alpha}commonpeptide antibodies recognize the {alpha} subunits of all G1{alpha} subtypes (G1–3). Immunoreactivity against {alpha}common and {alpha}i-common antibodies was found along the cell surface of cardiomyocytes and endothelial cells. No immunoreactivity was seen on sarcoplasmic reticulum membranes. T-tubule membranes showed a little reactivity. Distribution patterns obtained with {alpha}common and {alpha}i commonantibodies were identical. Immunogold was seen in cardiocytes from both atria and ventricles. In light-microscopical studies with peroxidase-conjugated secondary antibodies, heavy immunostaining was seen in SA and A V nodes.

Key Words: Immunocytochemistry • G-proteins • adenylyl cyclase localization • cardiomyocytes.


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