Copyright © 1992 by the European Society of Cardiology.
© 1992 The European Society of Cardiology
Ischaemic endothelial dysfunction after single or multidose cardioplegia
National Heart and Lung Institute Dovehouse Street, London SW3 6LY Harefield Hospital Harefield, Middlesex UB9 6JH, U.K
Correspondence: Mr Pankaj S. Mankad, Cardio thoracic Unit, The Hospital for Sick Children, Great Ormond Street, London WC1N 3JH, U.K.
Abstract
The effect of ischaemia, either alone or after a single or multidose infusion of St Thomas' Hospital cardioplegia solution (ST), on endothelial function was studied by examining its influence on 5-Hydroxytryptamine- (5-HT-) and nitroglycerine- (GTN-) induced coronary vasodilation in the isolated rat heart. Eighty rat hearts were perfused on a modified Langendorffpreparation. Thirty minutes of unprotected ischaemia (N=16) abolished the vasodilatory response to 5-HT but maintained the response to GTN (expressed as % of the control value 5-HT, pre 45.0 ±3.5, post 2.3 ±3.2, GTN, pre 42.3 ± 4.4, post 39.08 ± 4.0). The increase in coronary flow induced by either agent was unaltered following 30 or 60 min of ischaemia protected by a single dose ofST(N =16in each). Ninety minutes of ischaemia preceded by a single infusion ofST(N = 16) markedly reduced the 5-HT response but maintained the vasodilation induced by GTN (5-HT, pre 37±2.6, post 6.9±2.6, GTN, pre40.1 ±2.5, post 37±2.8). During 90 min ofischaemia, infusion of an additional dose of ST after 45 min (N =8) or two extra doses every 30 min (N = 8) maintained the 5-HT-induced increase in coronary flow (pre 39.5 ± 3.9, post 36.4 ± 3.5). It can be concluded that coronary vascular endothelium is more susceptible to ischaemic damage when compared with vascular smooth muscle. This results in early loss of endothelium-dependent vasodilation. Multidose infusion of St Thomas' Hospital cardioplegia solution is superior to a single infusion in protecting endothelial function following prolonged cardiac ischaemia.
Key Words: Endothelial function • ischaemia • St Thomas' solution • multidose cardioplegia • rat heart
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Parolari, P. Rubini, A. Cannata, L. Bonati, F. Alamanni, E. Tremoli, and P. Biglioli Endothelial damage during myocardial preservation and storage Ann. Thorac. Surg., February 1, 2002; 73(2): 682 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Gohra, H. Tsuboi, G. Sasaki, Y. Minami, K. Hamano, T. Katoh, Y. Fujimura, and K. Esato Effect of Cardioplegic Arrest and Reperfusion on Coronary Reserve and Autoregulation Ann. Thorac. Surg., September 1, 1996; 62(3): 744 - 749. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ogino, R. T. Smolenski, M. Zych, A.-M. L. Seymour, and M. H. Yacoub Influence of Preconditioning on Rat Heart Subjected to Prolonged Cardioplegic Arrest Ann. Thorac. Surg., August 1, 1996; 62(2): 469 - 474. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Saldanha and D. J. Hearse Cardioplegia and vascular injuryDissociation of the effects of ischemia from those of the cardioplegic solution J. Thorac. Cardiovasc. Surg., August 1, 1994; 108(2): 279 - 290. [Abstract] [Full Text]
|
|