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European Heart Journal 1993 14(7):930-935;
Copyright © 1993 by the European Society of Cardiology.
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© 1993 The European Society of Cardiology

Role of apolipoproteins A-I, A-II and C-I in cholesterol efflux from endothelial and smooth muscle cells

N. SAVION and S. KOTEV-EMETH

Maurice and Gabriela Goldschleger Eye Research Institute, Sackler Faculty of Medicine, Tel-Aviv University Tel-A viv, Israel

Received 3 May 1991; revised 12 February 1993; .

Correspondence: Prof Naphtali Savion, Maurice and Gabriela Goldschleger, Eye Research Institute, Sheba Medical Center, Tel Hashomer 52621, Israel

Abstract

The role of purified apolipoproteins A-I, A-II and C-fin the process of cholesterol efflux from cultured vascular endothelial and smooth muscle cells was studied [3H] Cholesterol pre-labelled cultures were exposed to serum-free medium supplemented with free apolipoproteins or with apolipoproteins and phosphatidylcholine liposomes and the cholesterol efflux from the cells was determined. Free apolipoproteins A-I and A-II supported cholesterol efflux from vascular endothelial and smooth muscle cells to a higher extent than apolipoprotein C-I. The ability of free apolipoproteins A-I and A-II to support cholesterol efflux was in correlation with their specific binding to the cultures, while no specfic binding of apolipoprotein C-I was detected. The association of apolipoprotein A-I with phosphatidylcholine liposomes resulted in a more than two-fold increase in cholesterol efflux compared to free apolipoprotein A-I, while association of apolipoproteins A-Il and C-I with phosphatidylcholine liposomes resulted in a very limited increase in cholesterol efflux above that achieved by the free apolipoproteins.

These results suggest that apolipoprotein A-I is involved in cholesterol efflux performed by high density lipoprotein. Furthermore, free apolipoproteins A-I and A-II, but not apolipoprotein C-I may take an active part in cholesterol efflux from endothelial and smooth muscle cells.

Key Words: Apolipoprotein A-I • apolipoprotein A-II • apolipoprotein C-I • vascular endothelium • smooth muscle cells • cholesterol efflux • high density lipoprotein • liposome


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