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European Heart Journal 1995 16(Supplement O):97-99; doi:10.1093/eurheartj/16.suppl_O.97
Copyright © 1995 by the European Society of Cardiology.
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© 1995 The European Society of Cardiology

Cytometric analysis of ventricular myocyte nuclei in idiopathic dilated cardiomyopathy: a tool for evaluation of disease progression?

K. M. Huhn*, B. Palcic*,{dagger}, J. E. Wilson* and B. M. McManus*,

* Department of Pathology and Laboratory Medicine, St. Paul's Hospital and the University of British Columbia, Vancouver B.C., Canada
{dagger} Cancer Imaging, British Columbia Cancer Centre, Vancouver B.C. Canada

Correspondence: Bruce M. McManus, MD, PhD, Department of Pathology and Laboratory Medicine, St. Paul's Hospital. 1081 Burrard Street, Vancouver, B.C V6Z 1Y6 Canada

The progression of idiopathic dilated cardiomyopathy (IDC) is governed by factors that remain obscure. The disease pathway toward cell degeneration or death results in irreversible myocyte change including nuclear cytometric alterations which may be evaluable and ultimately correlated with other measures of disease evolution.

Using a novel image cytometry system, we analysed differences in ventricular myocyte nuclear morphology and DNA content and distribution in right and left ventricular free wall myocardium and in ventricular septal myocardium from 11 normal and 13 IDC human autopsy hearts.

Nine morphological features of IDC myocyte nuclei differed significantly (P > 0.001) from normal. These were used to establish a classification matrix and cytometry-based assessment and allowed correct categorization of left and right ventricular and ventricular septal myocyte nuclei in concordance with their respective pathological diagnosis {i.e. normal or IDC) 71%, 81% and 77% of the time. Additionally, four photometric features were significantly different (P> 0.005) in IDC versus normal hearts, as were three discrete texture features (P > 0.001).

Thus, the spectrum of myocyte nuclei seen in IDC have highly characteristic and measurable morphologic, photometric and texture features. Our findings indicate the potential value of cytometry in the classification of myocytes with regards to a disease continuum and suggest its applicability in both clinical and experimental studies

Key Words: Idiopathic dilated cardiomyopathy • myocyte nuclei • cytometric analysis


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