Copyright © 1998 by the European Society of Cardiology.
Association between antibodies to heat shock protein 65 and coronary atherosclerosis. Possible mechanism of action ofHelicobacter pyloriand other bacterial infections in increasing cardiovascular risk
a Department of Medicine & Therapeutics, University of Glasgow, Western Infirmary, Glasgow, U.K.
b Department of Immunology, University of Glasgow, Western Infirmary, Glasgow, U.K.
accepted June 20, 1997
Introduction
There is growing evidence that the immune response is involved in atherosclerosis. Antibodies to heat shock protein 60/65 have been shown to be a risk factor for carotid atherosclerosis and been proposed as a diagnostic marker of atherosclerosis. In addition, it has been suggested that the immune response to heat shock protein 60/65 may be a link between exposure to microorganisms and increased cardiovascular risk.
Aims
(1) To investigate the association between anti-shock protein 65 titre and coronary atherosclerosis; (2) To assess whether anti-mhsp65 titre is a useful diagnostic marker of atherosclerosis; (3) To examine the influence of Helicobacter pylori infection on anti-heat shock protein 65 titre.
Methods and Results
In the first study we measured anti-heat shock protein 65 titres in 136 consecutive male subjects admitted for routine coronary angiography. Anti-heat shock protein 65 titres correlated with both the severity and extent of coronary atherosclerosis and the relationship remains statistically significant for the presence of atherosclerosis (P=0·012) after adjustment for possible confounding influences. However the association had in-sufficient sensitivity to be a useful clinical test. In the second study we recruited 100 patients with confirmed active H. pylori infection and double blindly randomized them to eradication therapy or placebo. Successful eradication of H. pylori led to a significant fall in anti-heat shock protein 65 titres (from a mean of 256·4AU.ml–1to 137·5AU.ml–1, P=0·033).
Conclusion
These results raise the possibility that exposure to H. pylori and other micro-organisms lead to an increased risk of clinically manifest coronary artery disease by an autoimmune process.
Key Words: Heat shock protein 65 • anti-mhsp65 • coronary atherosclerosis • Helicobacter pylori • auto-immunity
f1 Correspondence: Dr D. Birnie, Department of Medical Cardiology, Queen Elizabeth Building, Glasgow Royal Infirmary, Glasgow G31 2ER, U.K.
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