Copyright © 2003 by the European Society of Cardiology.
Clinical research
Autologous intramyocardial injection of cultured skeletal muscle-derived stem cells in patients with non-acute myocardial infarction
a Department of Cardiology and Cardiovascular Surgery, Clínica Universitaria, Universidad de Navarra, Pamplona, Spain
b Hematology and Cell Therapy Area, Clínica Universitaria, Universidad de Navarra, Pamplona, Spain
c Department of Nuclear Medicine, Clínica Universitaria, Universidad de Navarra, Pamplona, Spain
d Department of Hematology, Hospital Clínico Universitario de Salamanca, Salamanca, Spain
e Department of Cardiology and Cardiac Surgery, Hospital Clínico Universitario de Salamanca, Salamanca, Spain
* Correspondence to: Felipe Prósper, Hematology and Cell Therapy Area, Clínica Universitaria, Universidad de Navarra, Av Pio XII 36, Pamplona 31009, Spain. Tel: +34 948 255400; fax: +34 948 296500
E-mail address: fprosper{at}unav.es
Received 13 May 2003; revised 1 September 2003; accepted 11 September 2003
Abstract
Aim Experimental animal studies suggest that the use of skeletal myoblast in patients with myocardial infarction may result in improved cardiac function. The aim of the study was to assess the feasibility and safety of this therapy in patients with myocardial infarction.
Methods and results Twelve patients with old myocardial infarction and ischaemic coronary artery disease underwent treatment with coronary artery bypass surgery and intramyocardial injection of autologous skeletal myoblasts obtained from a muscle biopsy of vastus lateralis and cultured with autologous serum for 3 weeks. Global and regional cardiac function was assessed by 2D and ABD echocardiogram. 18F-FDG and 13N-ammonia PET studies were used to determine perfusion and viability. Left ventricular ejection fraction (LVEF) improved from 35.5±2.3% before surgery to 53.5±4.98% at 3 months (P=0.002). Echocardiography revealed a marked improvement in regional contractility in those cardiac segments treated with skeletal myoblast (wall motion score index 2.64±0.13 at baseline vs 1.64±0.16 at 3 months P=0.0001). Quantitative 18F-FDG PET studies showed a significant (P=0.012) increased in cardiac viability in the infarct zone 3 months after surgery. No statistically significant differences were found in 13N-ammonia PET studies. Skeletal myoblast implant was not associated with an increase in adverse events. No cardiac arrhythmias were detected during early follow-up.
Conclusions In patients with old myocardial infarction, treatment with skeletal myoblast in conjunction with coronary artery bypass is safe and feasible and is associated with an increased global and regional left ventricular function,improvement in the viability of cardiac tissue in the infarct area and no induction of arrhythmias.
Key Words: Non-acute myocardial infarction • Skeletal myoblasts • Coronary artery bypass
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Kendziorra, H. Barthel, S. Erbs, F. Emmrich, R. Hambrecht, G. Schuler, O. Sabri, and R. Kluge Effect of Progenitor Cells on Myocardial Perfusion and Metabolism in Patients After Recanalization of a Chronically Occluded Coronary Artery J. Nucl. Med., April 1, 2008; 49(4): 557 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Wang, W.-J. Cherng, N.-I Yang, L.-T. Kuo, C.-M. Hsu, H.-I Yeh, Y.-J. Lan, C.-H. Yeh, and W. L. Stanford Late-Outgrowth Endothelial Cells Attenuate Intimal Hyperplasia Contributed by Mesenchymal Stem Cells After Vascular Injury Arterioscler. Thromb. Vasc. Biol., January 1, 2008; 28(1): 54 - 60. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Gallegos and R. M. Bolman III Stem Cell Induced Regeneration of Myocardium Card. Surg. Adult, January 1, 2008; 3(2008): 1657 - 1668. [Full Text]
|
|
|
|
 |
|
 R. Martinez-Marmol, M. David, R. Sanches, M. Roura-Ferrer, N. Villalonga, E. Sorianello, S. M. Webb, A. Zorzano, A. Guma, C. Valenzuela, et al. Voltage-dependent Na+ channel phenotype changes in myoblasts. Consequences for cardiac repair Cardiovasc Res, December 1, 2007; 76(3): 430 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Prockop and S. D. Olson Clinical trials with adult stem/progenitor cells for tissue repair: let's not overlook some essential precautions Blood, April 15, 2007; 109(8): 3147 - 3151. [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Retuerto, J. T. Beckmann, J. Carbray, G. Patejunas, S. Sarateanu, B. J. Kane, B. Smulevitz, D. D. McPherson, and T. K. Rosengart Angiogenic pretreatment to enhance myocardial function after cellular cardiomyoplasty with skeletal myoblasts J. Thorac. Cardiovasc. Surg., February 1, 2007; 133(2): 478 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Zebedin, M. Mille, M. Speiser, T. Zarrabi, W. Sandtner, B. Latzenhofer, H. Todt, and K. Hilber C2C12 skeletal muscle cells adopt cardiac-like sodium current properties in a cardiac cell environment Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H439 - H450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Menasche, M. Desnos, and A. A. Hagege Myoblast transplantation during cardiac surgery Eur. Heart J. Suppl., December 1, 2006; 8(suppl_H): H52 - H56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Siminiak, E. Meliga, O. Jerzykowska, and P. W. Serruys Percutaneous transplantation of skeletal myoblast in the treatment of post-infarction injury Eur. Heart J. Suppl., December 1, 2006; 8(suppl_H): H57 - H64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. W. van Laake, R. Hassink, P. A. Doevendans, and C. Mummery Heart repair and stem cells J. Physiol., December 1, 2006; 577(2): 467 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Wu, Y. L. Liu, B. Zhou, and Z. C. Han Cellular therapy and myocardial tissue engineering: the role of adult stem and progenitor cells Eur. J. Cardiothorac. Surg., November 1, 2006; 30(5): 770 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ben-Dor, S. Fuchs, and R. Kornowski Potential Hazards and Technical Considerations Associated With Myocardial Cell Transplantation Protocols for Ischemic Myocardial Syndrome J. Am. Coll. Cardiol., October 17, 2006; 48(8): 1519 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Agbulut, M. Mazo, C. Bressolle, M. Gutierrez, K. Azarnoush, L. Sabbah, N. Niederlander, G. Abizanda, E. J. Andreu, B. Pelacho, et al. Can bone marrow-derived multipotent adult progenitor cells regenerate infarcted myocardium? Cardiovasc Res, October 1, 2006; 72(1): 175 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Caspi and L. Gepstein Stem cells for myocardial repair Eur. Heart J. Suppl., September 1, 2006; 8(suppl_E): E43 - E54. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Gavira, M. Perez-Ilzarbe, G. Abizanda, A. Garcia-Rodriguez, J. Orbe, J. A. Paramo, M. Belzunce, G. Rabago, J. Barba, J. Herreros, et al. A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction Cardiovasc Res, September 1, 2006; 71(4): 744 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Martens, M. Argenziano, and M. C. Oz New Technology for Surgical Coronary Revascularization Circulation, August 8, 2006; 114(6): 606 - 614. [Full Text] [PDF]
|
|
|
|
|
|
A. J. Boyle, S. P. Schulman, and J. M. Hare Stem Cell Therapy for Cardiac Repair: Ready for the Next Step Circulation, July 25, 2006; 114(4): 339 - 352. [Full Text] [PDF]
|
|
|
|
|
|
A. A. Hagege, J.-P. Marolleau, J.-T. Vilquin, A. Alheritiere, S. Peyrard, D. Duboc, E. Abergel, E. Messas, E. Mousseaux, K. Schwartz, et al. Skeletal Myoblast Transplantation in Ischemic Heart Failure: Long-Term Follow-Up of the First Phase I Cohort of Patients Circulation, July 4, 2006; 114(1_suppl): I-108 - I-113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Murry, H. Reinecke, and L. M. Pabon Regeneration Gaps: Observations on Stem Cells and Cardiac Repair J. Am. Coll. Cardiol., May 2, 2006; 47(9): 1777 - 1785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Serruys Fourth Annual American College of Cardiology International Lecture: A Journey in the Interventional Field J. Am. Coll. Cardiol., May 2, 2006; 47(9): 1754 - 1768. [Full Text] [PDF]
|
|
|
|
|
|
J. J. Gavira, J. Herreros, A. Perez, M. J. Garcia-Velloso, J. Barba, F. Martin-Herrero, C. Canizo, A. Martin-Arnau, J. M. Marti-Climent, M. Hernandez, et al. Autologous skeletal myoblast transplantation in patients with nonacute myocardial infarction: 1-year follow-up J. Thorac. Cardiovasc. Surg., April 1, 2006; 131(4): 799 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ye, H. K. Haider, and E. K. W. Sim Adult Stem Cells for Cardiac Repair: A Choice Between Skeletal Myoblasts and Bone Marrow Stem Cells Experimental Biology and Medicine, January 1, 2006; 231(1): 8 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Murry, L. J. Field, and P. Menasche Cell-Based Cardiac Repair: Reflections at the 10-Year Point Circulation, November 15, 2005; 112(20): 3174 - 3183. [Full Text] [PDF]
|
|
|
|
|
|
N. Dib, R. E. Michler, F. D. Pagani, S. Wright, D. J. Kereiakes, R. Lengerich, P. Binkley, D. Buchele, I. Anand, C. Swingen, et al. Safety and Feasibility of Autologous Myoblast Transplantation in Patients With Ischemic Cardiomyopathy: Four-Year Follow-Up Circulation, September 20, 2005; 112(12): 1748 - 1755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Valgimigli, G. M. Rigolin, C. Cittanti, P. Malagutti, S. Curello, G. Percoco, A. M. Bugli, M. D. Porta, L. Z. Bragotti, L. Ansani, et al. Use of granulocyte-colony stimulating factor during acute myocardial infarction to enhance bone marrow stem cell mobilization in humans: clinical and angiographic safety profile Eur. Heart J., September 2, 2005; 26(18): 1838 - 1845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C Chachques, C. Salanson-Lajos, P. Lajos, A. Shafy, A. Alshamry, and A. Carpentier Cellular Cardiomyoplasty for Myocardial Regeneration Asian Cardiovasc Thorac Ann, September 1, 2005; 13(3): 287 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hou, E. A.-S. Youssef, T. J. Brinton, P. Zhang, P. Rogers, E. T. Price, A. C. Yeung, B. H. Johnstone, P. G. Yock, and K. L. March Radiolabeled Cell Distribution After Intramyocardial, Intracoronary, and Interstitial Retrograde Coronary Venous Delivery: Implications for Current Clinical Trials Circulation, August 30, 2005; 112(9_suppl): I-150 - I-156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Siepe, C. Heilmann, P. von Samson, P. Menasche, and F. Beyersdorf Stem cell research and cell transplantation for myocardial regeneration Eur. J. Cardiothorac. Surg., August 1, 2005; 28(2): 318 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Brasselet, M. C. Morichetti, E. Messas, C. Carrion, A. Bissery, P. Bruneval, J.-T. Vilquin, A. Lafont, A. A. Hagege, P. Menasche, et al. Skeletal myoblast transplantation through a catheter-based coronary sinus approach: an effective means of improving function of infarcted myocardium Eur. Heart J., August 1, 2005; 26(15): 1551 - 1556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Siminiak, D. Fiszer, O. Jerzykowska, B. Grygielska, N. Rozwadowska, P. Kalmucki, and M. Kurpisz Percutaneous trans-coronary-venous transplantation of autologous skeletal myoblasts in the treatment of post-infarction myocardial contractility impairment: the POZNAN trial Eur. Heart J., June 2, 2005; 26(12): 1188 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fazel, G. H.L. Tang, D. Angoulvant, M. Cimini, R. D. Weisel, R.-K. Li, and T. M. Yau Current Status of Cellular Therapy for Ischemic Heart Disease Ann. Thorac. Surg., June 1, 2005; 79(6): S2238 - S2247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Schachinger and A. M. Zeiher Stem Cells and Cardiovascular and Renal Disease: Today and Tomorrow J. Am. Soc. Nephrol., March 1, 2005; 16(3_suppl_1): S2 - S6. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Sousa, M. A. Costa, E. M. Tuzcu, J. S. Yadav, and S. Ellis New Frontiers in Interventional Cardiology Circulation, February 8, 2005; 111(5): 671 - 681. [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Wollert and H. Drexler Clinical Applications of Stem Cells for the Heart Circ. Res., February 4, 2005; 96(2): 151 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Chachques, F. Duarte, B. Cattadori, A. Shafy, N. Lila, G. Chatellier, J.-N. Fabiani, and A. F. Carpentier Angiogenic growth factors and/or cellular therapy for myocardial regeneration: A comparative study J. Thorac. Cardiovasc. Surg., August 1, 2004; 128(2): 245 - 253. [Abstract] [Full Text] [PDF]
|
|