European Heart Journal Advance Access originally published online on February 13, 2007
European Heart Journal 2007 28(6):766-772; doi:10.1093/eurheartj/ehl509
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Cell isolation procedures matter: a comparison of different isolation protocols of bone marrow mononuclear cells used for cell therapy in patients with acute myocardial infarction
1 Department of Molecular Cardiology, Department of Internal Medicine III, University of Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
2 Institute for Transfusion Medicine and Immunohaematology, Red Cross Blood Donor Service Baden–Württemberg–Hessen, Frankfurt am Main, Germany
Received 10 June 2006; revised 1 July 2006; accepted 12 January 2007; online publish-ahead-of-print 13 February 2007.
* Corresponding author. Tel: +49 69 6301 6667; fax: +49 69 6301 7113. E-mail address: dimmeler{at}em.uni-frankfurt.de
See page 651 for the editorial comment on this article (doi:10.1093/eurheartj/ehm009)
Aim The recently published REPAIR-AMI and ASTAMI trial showed differences in contractile recovery of left ventricular function after infusion of bone marrow-derived cells in acute myocardial infarction. Since the trials used different protocols for cell isolation and storage (REPAIR-AMI: Ficoll, storage in X-vivo 10 medium plus serum; ASTAMI: Lymphoprep, storage in NaCl plus plasma), we compared the functional activity of BMC isolated by the two different protocols.
Methods and results The recovery of total cell number, colony-forming units (CFU), and the number of mesenchymal stem cells were significantly reduced to 77 ± 4%, 83 ± 16%, and 65 ± 15%, respectively, when using the ASTAMI protocol compared with the REPAIR protocol. The capacity of the isolated BMC to migrate in response to stromal cell-derived factor 1 (SDF-1) was profoundly reduced when using the ASTAMI cell isolation procedure (42 ± 8% and 78 ± 3% reduction in healthy and CAD-patient cells, respectively). Finally, infusion of BMC into a hindlimb ischaemia model demonstrated a significantly blunted blood-flow-recovery by BMC isolated with the ASTAMI protocol (54 ± 6% of the effect obtained by REPAIR cells). Comparison of the individual steps identified the use of NaCl and plasma for cell storage as major factors for functional impairment of the BMC.
Conclusion Cell isolation protocols have a major impact on the functional activity of bone marrow-derived progenitor cells. The assessment of cell number and viability may not entirely reflect the functional capacity of cells in vivo. Additional functional testing appears to be mandatory to assure proper cell function before embarking on clinical cell therapy trials.
Key Words: Cell therapy • BMC • Isolation protocols • Invasion capacity • REPAIR-AMI
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