European Heart Journal Advance Access originally published online on March 8, 2007
European Heart Journal 2007 28(9):1135-1142; doi:10.1093/eurheartj/ehl543
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Mutant ryanodine receptors in catecholaminergic polymorphic ventricular tachycardia generate delayed afterdepolarizations due to increased propensity to Ca2+ waves
1 Minerva Foundation Institute for Medical Research, FIN-00290, Helsinki, Finland
2 Department of Cardiology, Helsinki University Central Hospital, University of Helsinki, FIN-00290, Haartmaninkatu 4, PO Box 340, HUS 00029, Helsinki, Finland
3 Department of Medicine, University of Helsinki, FIN-00290, Helsinki, Finland
Received 7 October 2006; revised 9 January 2007; accepted 25 January 2007; online publish-ahead-of-print 8 March 2007.
* Corresponding author. Tel: +358 40 5245735; fax: +358 9 471 74574. E-mail address: Mika.Laine{at}hus.fi
See page 1054 for the editorial comment on this article (doi:10.1093/eurheartj/ehm068)
Aims: Mutations in cardiac ryanodine receptors (RyR2s) are linked to catecholaminergic polymorphic ventricular tachycardia (CPVT), characterized by risk of polymorphic ventricular tachyarrhythmias and sudden death during exercise. Arrhythmias are caused by gain-of-function defects in RyR2, but cellular arrhythmogenesis remains elusive.
Methods and results: We recorded endocardial monophasic action potentials (MAPs) at right ventricular septum in 15 CPVT patients with a RyR2 mutation (P2328S, Q4201R, and V4653F) and in 12 control subjects both at baseline and during epinephrine infusion (0.05 µg/kg/min). At baseline 3 and during epinephrine infusion, four CPVT patients, but none of the control subjects, showed delayed afterdepolarizations (DADs) occasionally coinciding with ventricular premature complexes. In order to study the underlying mechanisms, we expressed two types of mutant RyR2 (P2328S and V4653F) causing CPVT as well as wild-type RyR2 in HEK 293 cells. Confocal microscopy of Fluo-3 loaded cells transfected with any of the three RyR2s showed no spontaneous subcellular Ca2+ release events at baseline. Membrane permeable cAMP analogue (Dioctanoyl-cAMP) triggered subcellular Ca2+ release events as Ca2+ sparks and waves. Cells expressing mutant RyR2s showed spontaneous Ca2+ release events at lower concentrations of cAMP than cells transfected with wild-type RyR2.
Conclusion: CPVT patients show DADs coinciding with premature action potentials in MAP recordings. Expression studies suggest that DADs are caused by increased propensity of abnormal RyR2s to generate spontaneous Ca2+ waves in response to cAMP stimulation. Increased sensitivity of mutant RyR2s to cAMP may explain the occurrence of arrhythmias during exercise or emotional stress in CPVT.
Key Words: Arrhythmia • Calcium • Mutation • cAMP • HEK293 cells • Heart failure • Sudden death
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