European Heart Journal Advance Access published online on September 2, 2008
European Heart Journal, doi:10.1093/eurheartj/ehn382
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Interleukin-8 is increased in the membrane of circulating erythrocytes in patients with acute coronary syndrome
1 University Cardiology Department, Medical School, Democritus University of Thrace, Voulgaroktonou 23, 68100 Alexandroupolis, Evros, Greece
2 Biochemistry Department, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
3 Cardiovascular Biology Research Centre, St George’s Hospital, University of London, London, UK
4 Clinical Research Center, Biomedical Research Foundation, Academy of Athens, Athens, Greece
Received 19 February 2008; revised 22 July 2008; accepted 25 July 2008.
* Corresponding author. Tel: +30 25510 35596 (home)/+30 25510 76205 (office), Fax: +30 25510 76245, Email: dtziakas{at}med.duth.gr
Aims: Studies have shown that erythrocyte membranes are present within necrotic cores in atherosclerotic plaques, and that circulating erythrocytes in patients with acute coronary syndrome (ACS) have increased total cholesterol content (CEM). Interleukin-8 (IL-8) binds to erythrocytes and during intraplaque haemorrhage it is released into the plaque and thus may contribute to inflammatory cascade and atherosclerotic plaque instability. The present study was undertaken to test the hypothesis that erythrocyte membrane IL-8 is elevated in patients with ACS compared with those with chronic stable angina (CSA).
Methods and results: Consecutive patients who presented with CSA (n = 120, 92 men, 62 ± 9 years), ACS (n = 118, 90 men, 62 ± 10 years) or with chest pain who had normal coronary arteries (n = 36, 26 men, 60 ± 7 years), were studied prospectively. IL-8 concentrations in erythrocyte membranes (rIL-8) and in plasma (pIL-8), C-reactive protein (CRP) and CEM were measured. rIL-8 levels [mean ± 1 SD (standard deviation)] were higher in ACS (102.9 ± 70.1 pg/mL) compared with CSA (44.7 ± 22.8 pg/mL) (P < 0.001). No difference in pIL-8 levels between the two coronary artery disease groups was observed (P = 0.280). Serum CRP levels were correlated with rIL-8 levels (r = 0.294, P < 0.001); no association was found between CRP and pIL-8 levels (r = 0.025, P = 0.706). Further, rIL-8 had an independent association with ACS, when CRP and CEM were taken into consideration.
Conclusion: This study shows for the first time that rIL-8 content was significantly higher in ACS, compared with CSA. These findings endorse results from our previous studies suggesting that erythrocytes may play an important role in the development of unstable atherosclerotic plaque.
Key Words: Erythrocyte membranes • Interleukin-8 • Cholesterol content • Acute coronary syndrome • Chronic stable angina
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Barcelo, J. S. Perona, J. Prades, S. S. Funari, E. Gomez-Gracia, M. Conde, R. Estruch, and V. Ruiz-Gutierrez Mediterranean-Style Diet Effect on the Structural Properties of the Erythrocyte Cell Membrane of Hypertensive Patients: The Prevencion con Dieta Mediterranea Study Hypertension, November 1, 2009; 54(5): 1143 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Giugliano and E. Braunwald The Year in Non-ST-Segment Elevation Acute Coronary Syndrome. J. Am. Coll. Cardiol., October 13, 2009; 54(16): 1544 - 1555. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Apostolakis, K. Vogiatzi, V. Amanatidou, and D. A. Spandidos Interleukin 8 and cardiovascular disease Cardiovasc Res, August 4, 2009; (2009) cvp241v2. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pasterkamp and M. Daemen Circulating cells: the biofactory for markers of atherosclerotic disease Eur. Heart J., November 2, 2008; 29(22): 2701 - 2702. [Full Text] [PDF]
|
|