European Heart Journal Advance Access originally published online on May 22, 2006
European Heart Journal 2006 27(15):1868-1875; doi:10.1093/eurheartj/ehl013
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Tissue Doppler imaging predicts left ventricular dysfunction and mortality in a murine model of cardiac injury
1 Cardiac Ultrasound Laboratory
2 Cardiovascular Research Center, Cardiology Division of the Department of Medicine, Harvard Medical School USA
3 Institute for Technology Assessment, Massachusetts General Hospital USA
4 Division of Preventive Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, USA
5 Department of Cardiology, Hospital Louis Pradel, Lyon, France
Received 17 October 2005; revised 7 April 2006; accepted 13 April 2006; online publish-ahead-of-print 22 May 2006.
* Corresponding author. Tel: +1 617 7267686; fax: +1 617 7268383. E-mail address: marielle{at}crosbie.com
See page 1771 for the editorial comment on this article (doi:10.1093/eurheartj/ehl144)
Aims Currently available non-invasive imaging methods frequently fail to detect alterations in left ventricular (LV) function despite histological evidence of injury. Tissue Doppler imaging (TDI) can detect subtle LV dysfunction. The aim of this study was to investigate whether TDI indices can predict LV systolic dysfunction and mortality following exposure to doxorubicin (DOX) in mice.
Methods and results TDI-derived peak endocardial systolic velocity (VENDO) and strain rate (SR), as well as M-mode and two-dimensional indices of LV systolic function, were measured serially in mice after receiving DOX as a single dose (20 mg/kg). Haemodynamic measurements were obtained invasively before and at 1, 2, 4, and 5 days after the single DOX dose. Cardiac apoptosis was measured before and at 1 day after DOX. VENDO and SR decreased after 1 and 2 days, respectively, whereas changes in fractional shortening (FS) and LV ejection fraction (LVEF) were not detected before 5 days. The reduction in both VENDO and SR correlated with the decrease in dP/dtMAX, and the change in VENDO correlated with the early increase in cardiac cell apoptosis. In a subsequent experiment, DOX was administered at 4 mg/kg/week for 5 weeks, and LV function was followed serially for 16 weeks. In this chronic experiment, TDI indices decreased before FS and LVEF, correlated with late LV dysfunction, and predicted DOX-induced mortality.
Conclusion In a murine model of DOX-induced cardiac injury, TDI detects LV dysfunction prior to alterations in conventional echocardiographic indices and predicts mortality. This study suggests that TDI may be a reliable tool to detect early subtle changes in DOX-induced cardiac dysfunction.
Key Words: Echocardiography • Tissue Doppler imaging • Doxorubicin • Apoptosis • Heart failure
CiteULike 
Connotea 
Del.icio.us What's this?
Related articles in EHJ:
- Detection of subclinical LV dysfunction by tissue Doppler imaging
- Frank Weidemann and Joerg M. Strotmann
EHJ 2006 27: 1771-1772.[Extract] [FREE Full Text]
This article has been cited by other articles:
|
|
 |
|
  Y. Peng, Z. B. Popovic, N. Sopko, J. Drinko, Z. Zhang, J. D. Thomas, and M. S. Penn Speckle tracking echocardiography in the assessment of mouse models of cardiac dysfunction Am J Physiol Heart Circ Physiol, August 1, 2009; 297(2): H811 - H820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mukhopadhyay, M. Rajesh, S. Batkai, Y. Kashiwaya, G. Hasko, L. Liaudet, C. Szabo, and P. Pacher Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro Am J Physiol Heart Circ Physiol, May 1, 2009; 296(5): H1466 - H1483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. George, R. Shave, D. Oxborough, T. Cable, E. Dawson, N. Artis, D. Gaze, T. Hew-Butler, K. Sharwood, and T. Noakes Left ventricular wall segment motion after ultra-endurance exercise in humans assessed by myocardial speckle tracking Eur J Echocardiogr, March 1, 2009; 10(2): 238 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Derumeaux, F. Ichinose, M. J. Raher, J. G. Morgan, T. Coman, C. Lee, J. M. Cuesta, H. Thibault, K. D. Bloch, M. H. Picard, et al. Myocardial Alterations in Senescent Mice and Effect of Exercise Training: A Strain Rate Imaging Study Circ Cardiovasc Imaging, November 1, 2008; 1(3): 227 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Huebner, D. S. Jassal, O. Halevy, M. Pines, and J. E. Anderson Functional resolution of fibrosis in mdx mouse dystrophic heart and skeletal muscle by halofuginone Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1550 - H1561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mukhopadhyay, S. Batkai, M. Rajesh, N. Czifra, J. Harvey-White, G. Hasko, Z. Zsengeller, N. P. Gerard, L. Liaudet, G. Kunos, et al. Pharmacological Inhibition of CB1 Cannabinoid Receptor Protects Against Doxorubicin-Induced Cardiotoxicity J. Am. Coll. Cardiol., August 7, 2007; 50(6): 528 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Thibault, L. Gomez, E. Donal, G. Pontier, M. Scherrer-Crosbie, M. Ovize, and G. Derumeaux Acute myocardial infarction in mice: assessment of transmurality by strain rate imaging Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H496 - H502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ballo, A. Bocelli, and S. Mondillo Adaptive and Maladaptive Cardiac Hypertrophy: What Is the Effective Role of Heat Shock Transcription Factor 1? Circ. Res., February 16, 2007; 100(3): e45 - e46. [Full Text] [PDF]
|
|
|
|
 |
|
 T. G. Neilan and D. J. Fitzgerald Iloprost attenuates doxorubicin-induced cardiac injury in a murine model without compromising tumor suppression: reply Eur. Heart J., November 1, 2006; 27(21): 2611 - 2611. [Full Text] [PDF]
|
|
|
|
|
|
F. Weidemann and J. M. Strotmann Detection of subclinical LV dysfunction by tissue Doppler imaging Eur. Heart J., August 1, 2006; 27(15): 1771 - 1772. [Full Text] [PDF]
|
|