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European Heart Journal Advance Access published online on December 20, 2007
European Heart Journal, doi:10.1093/eurheartj/ehm588
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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2008. For permissions please email: journals.permissions@oxfordjournals.org.

Non-invasive detection and quantification of acute myocardial infarction in rabbits using mono-[123I]iodohypericin µSPECT

Humphrey Fonge1, Kathleen Vunckx2, Huaijun Wang3, Yuanbo Feng3, Luc Mortelmans2, Johan Nuyts2, Guy Bormans1, Alfons Verbruggen1,* and Yicheng Ni3

1 Laboratory for Radiopharmacy, Faculty of Pharmaceutical Sciences, K.U. Leuven, O&N 2, Box 821, Herestraat 49, BE-3000 Leuven, Belgium
2 Department of Nuclear Medicine, University Hospital, K.U. Leuven, Herestraat 49, BE-3000 Leuven, Belgium
3 Department of Radiology, University Hospital, K.U. Leuven, Herestraat 49, BE-3000 Leuven, Belgium

Received 28 May 2007; revised 17 November 2007; accepted 22 November 2007.

* Corresponding author. Tel: +32 16 33 04 46, Fax: +32 16 33 04 49. Email: alfons.verbruggen{at}pharm.kuleuven.be

Aims: Mono-[123I]iodohypericin ([123I]MIH) has been reported to have high avidity for necrosis. In the present study, by using rabbit models of acute myocardial infarction, we explored the suitability of [123I]MIH micro single photon emission computed tomography (µSPECT) for non-invasive visualization of myocardial infarcts in comparison with [13N]ammonia micro positron emission tomography (µPET) imaging, postmortem histomorphometry, and [123I]MIH autoradiography.

Methods and results: Fourteen rabbits were divided into four groups. The left circumflex coronary artery was permanently occluded in group A (n = 3), reperfused by releasing the ligature after 15 min in group B (n = 3) or 90 min in group C (n = 6), or not occluded in group D (n = 2). Animals received [13N]ammonia µPET perfusion imaging 18 h after infarct induction followed by µSPECT imaging at 2–3.5, 9–11, and 22–24 h post injection (p.i.) of [123I]MIH. The cardiac images were assembled into polar maps for assessment of tracer uptake. Animals were sacrificed and the excised heart was sliced for autoradiography, triphenyl tetrazolium chloride, and haematoxylin–eosin staining. Using [123I]MIH µSPECT, infarcts were well delineated at 9 h p.i. Mean µSPECT infarct size was 38.8 and 32.7% of left ventricular area for groups A and C, respectively, whereas group B showed low uptake of [123I]MIH. Highest mean infarct/viable tissue activity ratio of 61/1 was obtained by autoradiography in group C animals at 24 h p.i.

Conclusion: The study indicates the suitability of [123I]MIH for in vivo visualization of myocardial infarcts.

Key Words: Necrosis • Mono-[123I]iodohypericin • µSPECT • Infarct avidity • Cardiac imaging

This paper was guest edited by Prof. Dr Markus Schwaiger, Klinik r.d. Isar der TU Muenchen, Nuklearmed. Klinik u. Poliklinik, Munich, Germany


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